Search on: MICROSCOPY, FLUORESCENCE, MULTIPHOTON 
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Descriptor English:   Microscopy, Fluorescence, Multiphoton 
Descriptor Spanish:   Microscopía de Fluorescencia por Excitación Multifotónica 
Descriptor Portuguese:   Microscopia de Fluorescência por Excitação Multifotônica 
Synonyms English:   Microscopy, Multiphoton Fluorescence
Fluorescence Microscopy, Multiphoton
Multiphoton Fluorescence Microscopy  
Tree Number:   E01.370.350.515.458.500
E05.595.458.500
Definition English:   Fluorescence microscopy utilizing multiple low-energy photons to produce the excitation event of the fluorophore. Multiphoton microscopes have a simplified optical path in the emission side due to the lack of an emission pinhole, which is necessary with normal confocal microscopes. Ultimately this allows spatial isolation of the excitation event, enabling deeper imaging into optically thick tissue, while restricting photobleaching and phototoxicity to the area being imaged. 
History Note English:   2003 
Allowable Qualifiers English:  
AE adverse effects CL classification
CT contraindications EC economics
ES ethics HI history
IS instrumentation MT methods
MO mortality NU nursing
PX psychology ST standards
SN statistics & numerical data TD trends
UT utilization VE veterinary
Record Number:   37594 
Unique Identifier:   D036641 

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